It was shown previously' that in a cell-free system of protein synthesis consisting of purified Escherichia coli ribosomes and Lactobacillus arabinosus supernatant,
نویسندگان
چکیده
chromasia was not limited to mammalian cells but occurred also in primitive eukaryotic organisms. A model is proposed which postulates that the cell membrane is permeable to the substrate, a nonpolar compound, and less permeable to the polar product, fluorescein. Quantitative studies of fluorochromasia with fluorescein analogues, both at the population and single cell level, are consistent with this model.
منابع مشابه
Efficacy of lyophilized cell-free supernatant of Lactobacillus salivarius (Ls-BU2) on Escherichia coli and shelf life of ground beef
In the present study, the effect of different concentrations of cell-free supernatant (CFS; 10.00 and 35.00 mg g-1) of Lactobacillus salivarius (Ls-BU2) on chemical, microbial and sensorial specifications of ground beef stored under the refrigerated condition was investigated. The antibacterial activity of CFS on Escherichia coli was also assessed. According to agar...
متن کاملDirection of reading of the genetic message.
The assembly of polypeptide chains during protein biosynthesis is believed to proceed from the NHz-terminal through the COOH-terminal amino acid (l-4). Hence, the most direct method for ascertaining the direction in which the genetic message is read is to determine the end location of a given amino acid in polypeptide chains synthesized in a cell-free system under the direction of synthetic pol...
متن کاملAntibiofilm potential of Lactobacillus plantarum spp. cell free supernatant (CFS) against multidrug resistant bacterial pathogens
Biofilm formation is a major determinant factor in development of bacterial infections. In addition, bacteria embedded in a biofilm are more resistant to antimicrobials and thus the ability of bacteria to persist and grow in a biofilm seems to be the major factor for pathogenesis and therapeutic failure. In the current study, a Lactobacillus plantarum spp was isolated from Siahmazgi cheese, tra...
متن کاملRole of bacterial ribosomes in barotolerance.
The effects of high hydrostatic pressures on protein synthesis by whole cells and cell free preparations of Escherichia coli, Pseudomonas fluorescens, and Pseudomonas bathycetes were determined. Actively growing cells of P. bathycetes and P. fluorescens were less sensitive than were E. coli cells. Protein synthesis by cell free preparations of E. coli and P. fluorescens showed the same extent o...
متن کاملTetracycline Inhibition of Cell-Free Protein Synthesis
DAY, L. E. (Chas. Pfizer & Co., Inc., Groton, Conn.). Tetracycline inhibition of cell-free protein synthesis. II. Effect of the binding of tetracycline to the components of the system. J. Bacteriol. 92:197-203. 1966.-When tetracycline, an inhibitor of cell-free protein synthesis, was preincubated with each component of the Escherichia coli cell-free system, i.e., ribosomes, soluble ribonucleic ...
متن کامل